
Proceedings Paper
Time-resolved confocal fluorescence spectroscopy reveals the structure and metabolic state of epithelial tissueFormat | Member Price | Non-Member Price |
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Paper Abstract
Autofluorescence spectroscopy has been a widely explored technique for in vivo and noninvasive diagnosis of pre-cancer
lesions in epithelium where 90% cancers originate. For extracting more accurate fluorescence information for cancer
diagnosis, depth-resolved fluorescence measurements are crucial to assess NADH and FAD in non-keratinized epithelial
layer and collagen in stromal layer, respectively. In this study, we achieved the depth-resolved fluorescence spectral
measurements of squamous epithelial tissue based on confocal technique. We found that in non-keratinized epithelial
layer the fluorescence signals excited at 405 nm were the combination of NADH and FAD fluorescence and could be
used for evaluating the redox ratio. Moreover, we found that confocal time-resolved autofluorescence measurements of
epithelial tissue with 405 nm excitations could provide the information on the layered tissue structure. All depth-resolved
autofluorescence decays were accurately fitted with a dual-exponential function consisting of a short lifetime (0.4 ~ 0.6
ns) and a long lifetime (3 ~ 4 ns) components. The short lifetime component dominated the decay of non-keratinzied
epithelial fluorescence while the decay of the signals from keratinized epithelium and stroma were mainly determined by
the long lifetime component. The ratio of the amplitudes of two components could be used to differentiate the layered
structure of epithelial tissue. In general, the results in this study demonstrated that the combined depth- and timeresolved
fluorescence measurements can produce the information on the layered structure and localized biochemistry of
epithelial tissue for the diagnosis of tissue pathology.
Paper Details
Date Published: 6 February 2007
PDF: 5 pages
Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643013 (6 February 2007); doi: 10.1117/12.702450
Published in SPIE Proceedings Vol. 6430:
Advanced Biomedical and Clinical Diagnostic Systems V
Ramesh Raghavachari; Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)
PDF: 5 pages
Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643013 (6 February 2007); doi: 10.1117/12.702450
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Wei Zheng, Hong Kong Univ. of Science and Technology (Hong Kong China)
Wei Zheng, Hong Kong Univ. of Science and Technology (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Published in SPIE Proceedings Vol. 6430:
Advanced Biomedical and Clinical Diagnostic Systems V
Ramesh Raghavachari; Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)
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