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Proceedings Paper

Confocal time-resolved fluorescence anisotropy imaging
Author(s): Arjen N. Bader; Erik G. Hofman; Paul van Bergen en Henegouwen; Hans C. Gerritsen
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Paper Abstract

A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be made between the anisotropy in the plasma membrane and in the interior of the cell.

Paper Details

Date Published: 19 February 2007
PDF: 9 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (19 February 2007); doi: 10.1117/12.702031
Show Author Affiliations
Arjen N. Bader, Utrecht Univ. (Netherlands)
Erik G. Hofman, Utrecht Univ. (Netherlands)
Paul van Bergen en Henegouwen, Utrecht Univ. (Netherlands)
Hans C. Gerritsen, Utrecht Univ. (Netherlands)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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