
Proceedings Paper
Confocal time-resolved fluorescence anisotropy imagingFormat | Member Price | Non-Member Price |
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Paper Abstract
A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning
microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally
developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with
the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time
resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be
made between the anisotropy in the plasma membrane and in the interior of the cell.
Paper Details
Date Published: 19 February 2007
PDF: 9 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (19 February 2007); doi: 10.1117/12.702031
Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)
PDF: 9 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (19 February 2007); doi: 10.1117/12.702031
Show Author Affiliations
Arjen N. Bader, Utrecht Univ. (Netherlands)
Erik G. Hofman, Utrecht Univ. (Netherlands)
Erik G. Hofman, Utrecht Univ. (Netherlands)
Paul van Bergen en Henegouwen, Utrecht Univ. (Netherlands)
Hans C. Gerritsen, Utrecht Univ. (Netherlands)
Hans C. Gerritsen, Utrecht Univ. (Netherlands)
Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)
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