
Proceedings Paper
Laser scanning confocal microscopy and laser tweezers based experiments to understand dentine-bacteria interactionsFormat | Member Price | Non-Member Price |
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Paper Abstract
Failure of endodontic treatment is commonly due to Enterococcal infection. In this study influence of chemical
treatments of type-I collagen membrane by chemical agents commonly used in endodontic treatment on Enterococcus
faecalis cell adherence was evaluated. In order to determine the change in number of adhering bacteria after chemical
treatment, confocal laser scanning microscopy was used. For this, overnight culture of E faecalis in All Culture broth
was applied to chemically treated type-I collagen membrane. It was found that Ca(OH)2 treated groups had statistically
significant (p value=0.05) increase in population of bacteria adherence. The change in adhesion force between bacteria
and collagen was determined by using optical tweezers (1064 nm). For this experiment, Type-I collagen membrane was
soaked for 5 mins in a media that contained 50% all culture media and 50% saturated Ca(OH)2 . The membrane was
spread on the coverslip, on which diluted bacterial suspension was added. The force of laser tweezers on the bacteria was
estimated at different trap power levels using viscous drag method and trapping stiffness was calculated using
Equipartition theorem method. Presence of Ca(OH)2 was found to increase the cell-substrate adherence force from
0.38pN to >2.1pN. Together, these experiments show that it was highly probable that the increase in adherence to
collagen was due to a stronger adhesion in the presence of Ca (OH)2.
Paper Details
Date Published: 8 March 2007
PDF: 11 pages
Proc. SPIE 6425, Lasers in Dentistry XIII, 642504 (8 March 2007); doi: 10.1117/12.701784
Published in SPIE Proceedings Vol. 6425:
Lasers in Dentistry XIII
Peter Rechmann D.D.S.; Daniel Fried, Editor(s)
PDF: 11 pages
Proc. SPIE 6425, Lasers in Dentistry XIII, 642504 (8 March 2007); doi: 10.1117/12.701784
Show Author Affiliations
Sum Chee Peng, National Univ. of Singapore (Singapore)
Samarendra Mohanty, Raja Ramanna Ctr. for Advanced Technology (India)
Samarendra Mohanty, Raja Ramanna Ctr. for Advanced Technology (India)
P. K. Gupta, Raja Ramanna Ctr. for Advanced Technology (India)
Anil Kishen, National Univ. of Singapore (Singapore)
Anil Kishen, National Univ. of Singapore (Singapore)
Published in SPIE Proceedings Vol. 6425:
Lasers in Dentistry XIII
Peter Rechmann D.D.S.; Daniel Fried, Editor(s)
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