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Hardware, software, and scanning issues encountered during small animal imaging of photodynamic therapy in the athymic nude rat
Author(s): Nathan Cross; Rahul Sharma; Davood Varghai M.D.; Chandra Spring-Robinson; Nancy L. Oleinick; Raymond F. Muzic Jr.; David Dean
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Paper Abstract

Small animal imaging devices are now commonly used to study gene activation and model the effects of potential therapies. We are attempting to develop a protocol that non-invasively tracks the affect of Pc 4-mediated photodynamic therapy (PDT) in a human glioma model using structural image data from micro-CT and/or micro-MR scanning and functional data from 18F-fluorodeoxy-glucose (18F-FDG) micro-PET imaging. Methods: Athymic nude rat U87-derived glioma was imaged by micro-PET and either micro-CT or micro-MR prior to Pc 4-PDT. Difficulty insuring animal anesthesia and anatomic position during the micro-PET, micro-CT, and micro-MR scans required adaptation of the scanning bed hardware. Following Pc 4-PDT the animals were again 18F-FDG micro-PET scanned, euthanized one day later, and their brains were explanted and prepared for H&E histology. Histology provided the gold standard for tumor location and necrosis. The tumor and surrounding brain functional and structural image data were then isolated and coregistered. Results: Surprisingly, both the non-PDT and PDT groups showed an increase in tumor functional activity when we expected this signal to disappear in the group receiving PDT. Co-registration of the functional and structural image data was done manually. Discussion: As expected, micro-MR imaging provided better structural discrimination of the brain tumor than micro-CT. Contrary to expectations, in our preliminary analysis 18F-FDG micro-PET imaging does not readily discriminate the U87 tumors that received Pc 4-PDT. We continue to investigate the utility of micro-PET and other methods of functional imaging to remotely detect the specificity and sensitivity of Pc 4-PDT in deeply placed tumors.

Paper Details

Date Published: 23 March 2007
PDF: 7 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242K (23 March 2007); doi: 10.1117/12.701642
Show Author Affiliations
Nathan Cross, Case Western Reserve Univ. (United States)
Rahul Sharma, Case Western Reserve Univ. (United States)
Davood Varghai M.D., Case Western Reserve Univ. (United States)
Chandra Spring-Robinson, Case Western Reserve Univ. (United States)
Nancy L. Oleinick, Case Western Reserve Univ. (United States)
Raymond F. Muzic Jr., Case Western Reserve Univ. (United States)
David Dean, Case Western Reserve Univ. (United States)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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