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Proceedings Paper

Fourier transform infrared spectroscopic analysis of cell differentiation
Author(s): Katsunori Ishii; Akinori Kimura; Toshihiro Kushibiki; Kunio Awazu
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Paper Abstract

Stem cells and its differentiations have got a lot of attentions in regenerative medicine. The process of differentiations, the formation of tissues, has become better understood by the study using a lot of cell types progressively. These studies of cells and tissue dynamics at molecular levels are carried out through various approaches like histochemical methods, application of molecular biology and immunology. However, in case of using regenerative sources (cells, tissues and biomaterials etc.) clinically, they are measured and quality-controlled by non-invasive methods from the view point of safety. Recently, the use of Fourier Transform Infrared spectroscopy (FT-IR) has been used to monitor biochemical changes in cells, and has gained considerable importance. The objective of this study is to establish the infrared spectroscopy of cell differentiation as a quality control of cell sources for regenerative medicine. In the present study, as a basic study, we examined the adipose differentiation kinetics of preadipocyte (3T3-L1) and the osteoblast differentiation kinetics of bone marrow mesenchymal stem cells (Kusa-A1) to analyze the infrared absorption spectra. As a result, we achieved to analyze the adipose differentiation kinetics using the infrared absorption peak at 1739 cm-1 derived from ester bonds of triglyceride and osteoblast differentiation kinetics using the infrared absorption peak at 1030 cm-1 derived from phosphate groups of calcium phosphate.

Paper Details

Date Published: 7 February 2007
PDF: 7 pages
Proc. SPIE 6439, Optics in Tissue Engineering and Regenerative Medicine, 64390I (7 February 2007); doi: 10.1117/12.701535
Show Author Affiliations
Katsunori Ishii, Osaka Univ. (Japan)
Akinori Kimura, Osaka Univ. (Japan)
Toshihiro Kushibiki, Osaka Univ. (Japan)
Japan Science and Technology Organization (Japan)
Kunio Awazu, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 6439:
Optics in Tissue Engineering and Regenerative Medicine
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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