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Proceedings Paper

Characterization of scaffold architecture by optical coherence tomography
Author(s): Ying Yang; Pierre O. Bagnaninchi; Cassilda Cunha-Reis; Halil M. Aydin; Erhan Piskin; Alicia El Haj
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Paper Abstract

Cells, scaffold and culture environment are the three essential elements in engineering tissue constructs. Among these elements, the scaffold plays a critical role in converting cells into tissue since it provides a template and space for cells to grow and produce the desired matrix. Scaffolds are usually fabricated into three-dimensional blocks from biodegradable polymers with different internal architectures, for instance they are with fibrous or porous structures. The mechanical properties and nutrient diffusion ability of scaffolds are highly dependent on their internal structure. The biodegradable feature of scaffolds leads to a dramatic change in their microstructure during in vitro culture or after implantation. In this study, we explore optical coherence tomography (OCT) as a potential tool to characterize architecture of scaffolds including porosity, pore distribution and interconnectivity. This instrument is a fibre based time domain OCT equipped with a 1300 nm superluminescent diode, with a bandwidth of 52 nm and a free space resolution of 16x16x14 μm. Two model scaffold systems have been investigated. One was porous poly(lactide) scaffold fabricated by solvent-evaporation and salt leaching technique with dual poregens. Another was fibrous chitosan scaffold produced by wet spinning. Variations of scaffolds architecture, in term of porosity and interconnectivity, with different fabrication conditions could be quantified with the help of a commercial software (Volocity, Improvision). This study demonstrated that OCT can be used as a tool to guide scaffold fabrication and optimise their internal structure. Moreover, it can be used as on-line monitoring for scaffold degradation in various culture conditions.

Paper Details

Date Published: 8 February 2007
PDF: 9 pages
Proc. SPIE 6439, Optics in Tissue Engineering and Regenerative Medicine, 64390G (8 February 2007); doi: 10.1117/12.701103
Show Author Affiliations
Ying Yang, Keele Univ. (United Kingdom)
Pierre O. Bagnaninchi, Keele Univ. (United Kingdom)
Cassilda Cunha-Reis, Keele Univ. (United Kingdom)
Halil M. Aydin, Hacettepe Univ. (Turkey)
Erhan Piskin, Hacettepe Univ. (Turkey)
Alicia El Haj, Keele Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 6439:
Optics in Tissue Engineering and Regenerative Medicine
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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