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Proceedings Paper

Erythrocytes volume and refractive index measurement with a digital holographic microscope
Author(s): B. Rappaz; A. Barbul; F. Charrière; J. Kühn; P. Marquet; R. Korenstein; C. Depeursinge; P. Magistretti
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Paper Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.

Paper Details

Date Published: 14 February 2007
PDF: 5 pages
Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644509 (14 February 2007); doi: 10.1117/12.700463
Show Author Affiliations
B. Rappaz, Brain Mind Institute, EPFL (Switzerland)
A. Barbul, Sackler Faculty of Medicine (Israel)
F. Charrière, Imaging and Applied Optics Institute, EPFL (Switzerland)
J. Kühn, Imaging and Applied Optics Institute, EPFL (Switzerland)
P. Marquet, Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)
R. Korenstein, Sackler Faculty of Medicine (Israel)
C. Depeursinge, Imaging and Applied Optics Institute, EPFL (Switzerland)
P. Magistretti, Brain Mind Institute, EPFL (Switzerland)
Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)

Published in SPIE Proceedings Vol. 6445:
Optical Diagnostics and Sensing VII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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