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Proceedings Paper

High-speed line scanning confocal microscope for biological imaging
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Paper Abstract

We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

Paper Details

Date Published: 14 February 2007
PDF: 6 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644318 (14 February 2007); doi: 10.1117/12.700460
Show Author Affiliations
Seung-Hwan Jung, Yonsei Univ. (South Korea)
Chang-Keun Kim, Yonsei Univ. (South Korea)
Sung-Bin Ju, Yonsei Univ. (South Korea)
Yong-Jin Cho, Yonsei Univ. (South Korea)
Hyun-Woo Jeong, Yonsei Univ. (South Korea)
Beop-Min Kim, Yonsei Univ. (South Korea)

Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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