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Proceedings Paper

Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence
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Paper Abstract

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

Paper Details

Date Published: 14 February 2007
PDF: 6 pages
Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 64500O (14 February 2007); doi: 10.1117/12.700343
Show Author Affiliations
Soon Joon Yoon, Yonsei Univ. (South Korea)
Donghyun Kim, Yonsei Univ. (South Korea)

Published in SPIE Proceedings Vol. 6450:
Plasmonics in Biology and Medicine IV
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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