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Proceedings Paper

Time-resolved multiphoton imaging of basal cell carcinoma
Author(s): R. Cicchi; S. Sestini; V. De Giorgi; D. Stambouli; P. Carli; D. Massi; F. S. Pavone
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Paper Abstract

We investigated human cutaneous basal cell carcinoma ex-vivo samples by combined time resolved two photon intrinsic fluorescence and second harmonic generation microscopy. Morphological and spectroscopic differences were found between malignant skin and corresponding healthy skin tissues. In comparison with normal healthy skin, cancer tissue showed a different morphology and a mean fluorescence lifetime distribution slightly shifted towards higher values. Topical application of delta-aminolevulinic acid to the lesion four hours before excision resulted in an enhancement of the fluorescence signal arising from malignant tissue, due to the accumulation of protoporphyrines inside tumor cells. Contrast enhancement was prevalent at tumor borders by both two photon fluorescence microscopy and fluorescence lifetime imaging. Fluorescence-based images showed a good correlation with conventional histopathological analysis, thereby supporting the diagnostic accuracy of this novel method. Combined morphological and lifetime analysis in the study of ex-vivo skin samples discriminated benign from malignant tissues, thus offering a reliable, non-invasive tool for the in-vivo analysis of inflammatory and neoplastic skin lesions.

Paper Details

Date Published: 10 February 2007
PDF: 9 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421I (10 February 2007); doi: 10.1117/12.700216
Show Author Affiliations
R. Cicchi, Univ. degli Studi di Firenze (Italy)
S. Sestini, Univ. degli Studi di Firenze (Italy)
V. De Giorgi, Univ. degli Studi di Firenze (Italy)
D. Stambouli, Univ. degli Studi di Firenze (Italy)
P. Carli, Univ. degli Studi di Firenze (Italy)
D. Massi, Univ. degli Studi di Firenze (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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