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Proceedings Paper

Refractive multiple optical tweezers for parallel biochemical analysis in micro-fluidics
Author(s): Fabrice Merenda; Johann Rohner; Pedro Pascoal; Jean-Marc Fournier; Horst Vogel; René P. Salathé
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Paper Abstract

We present a multiple laser tweezers system based on refractive optics. The system produces an array of 100 optical traps thanks to a refractive microlens array, whose focal plane is imaged into the focal plane of a high-NA microscope objective. This refractive multi-tweezers system is combined to micro-fluidics, aiming at performing simultaneous biochemical reactions on ensembles of free floating objects. Micro-fluidics allows both transporting the particles to the trapping area, and conveying biochemical reagents to the trapped particles. Parallel trapping in micro-fluidics is achieved with polystyrene beads as well as with native vesicles produced from mammalian cells. The traps can hold objects against fluid flows exceeding 100 micrometers per second. Parallel fluorescence excitation and detection on the ensemble of trapped particles is also demonstrated. Additionally, the system is capable of selectively and individually releasing particles from the tweezers array using a complementary steerable laser beam. Strategies for high-yield particle capture and individual particle release in a micro-fluidic environment are discussed. A comparison with diffractive optical tweezers enhances the pros and cons of refractive systems.

Paper Details

Date Published: 9 February 2007
PDF: 9 pages
Proc. SPIE 6483, Complex Light and Optical Forces, 64830A (9 February 2007); doi: 10.1117/12.700201
Show Author Affiliations
Fabrice Merenda, École Polytechnique Fédérale de Lausanne (Switzerland)
Johann Rohner, École Polytechnique Fédérale de Lausanne (Switzerland)
Pedro Pascoal, École Polytechnique Fédérale de Lausanne (Switzerland)
Jean-Marc Fournier, École Polytechnique Fédérale de Lausanne (Switzerland)
Horst Vogel, École Polytechnique Fédérale de Lausanne (Switzerland)
René P. Salathé, École Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 6483:
Complex Light and Optical Forces
David L. Andrews; Enrique J. Galvez; Gerard Nienhuis, Editor(s)

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