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Proceedings Paper

The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II) and Talaporfin sodium with pulsed excitation
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Paper Abstract

In order to investigate the interaction between the triplet state T1 and ground state oxygen 3O2 during pulsed excitation photodynamic therapy (PDT), we measured the phosphorescence and singlet oxygen 1O2 fluorescence time-resolved waveform. The phosphorescence time-resolved waveform from clinical photosensitizers has not been obtained because this signal was buried in the photosensitizer fluorescence. We constructed the experimental setup with a spectral and temporal filter to select the phosphorescence signals from the Photofrin(II)(R) and Talaporfin sodium solution. The lifetimes and spectrums of the measured luminescence coincided with the phosphorescence characteristics, respectively. We obtained the phosphorescence time-resolved waveforms from the clinical photosensitizer solutions successfully. The 1O2 fluorescence time-resolved waveforms from these photosensitizers were measured with an IR-PMT with a photon counter. The fluorescence time-resolved waveforms of each photosensitizer were also obtained by the authors. We could consequently describe sequential generation of three time-resolved waveforms throughout the photosensitive reaction in the clinical photosensitizers. We think we may evaluate the photoseisitizer characteristics by these waveforms.

Paper Details

Date Published: 28 February 2007
PDF: 8 pages
Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 642715 (28 February 2007); doi: 10.1117/12.699857
Show Author Affiliations
Shiho Hakomori, Keio Univ. (Japan)
Sayaka Ohmori, Keio Univ. (Japan)
Kensuke Masuda, Keio Univ. (Japan)
Kojiro Yamamoto, Keio Univ. (Japan)
Tsunenori Arai, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 6427:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI
David Kessel, Editor(s)

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