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Proceedings Paper

Luminescent up-converting nanocrystals for in vivo imaging
Author(s): Isabelle Texier; Emilie Heinrich; Michel Berger; O. Tillement; C. Louis; Philippe Peltié
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Paper Abstract

Luminescent nanoparticles are increasingly used as emitting labels in the design of fluorescence imaging probes, because of their outstanding optical properties, such as in the case of quantum dots, or their role in nano-assembling different functionalities, such as imaging, drug delivery, and therapy. In this work, the potentiality of up-converting nano-crystals for non invasive fluorescence imaging of small animal is explored. These up-converting nano-crystals are lanthanide doped oxide matrices, such as Y2O3:Er,Yb compositions. They are produced by a bottom-up process and a thermal treatment. They are functionalized by the coating of a thin polysiloxane shell layer, which can be further derivatized in order to graft biomolecules such as antibodies, peptides, or DNA. Contrary to classical luminescent particles for which light emission occurs at a wavelength superior to that of excitation, these materials emit at 564 and 661 nm upon excitation at 980 or 815 nm. These unique emission properties, due to a multi-photonic process, can allow imaging without any auto-fluorescence from the tissues in the wavelength detection window. Experiments in phantoms mimicking the optical absorption and diffusion of tissues show that these crystals can be detected 4 mm deep at a 10 mg/mL concentration. Luminescence measurements in mouse and the potentiality of these nano-crystals for in vivo imaging are discussed.

Paper Details

Date Published: 13 February 2007
PDF: 11 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490D (13 February 2007); doi: 10.1117/12.699848
Show Author Affiliations
Isabelle Texier, LETI/DTBS CEA Grenoble (France)
Emilie Heinrich, LETI/DTBS CEA Grenoble (France)
Michel Berger, LETI/DTBS CEA Grenoble (France)
O. Tillement, Nano-H S.A.S. (France)
C. Louis, Nano-H S.A.S. (France)
Philippe Peltié, LETI/DTBS CEA Grenoble (France)

Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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