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Proceedings Paper

High-resolution surface plasmon resonance biosensing system for glucose concentration detecting
Author(s): Xian Huang; Dachao Li; Haixia Yu; Fuxiang Huang; Xiaotang Hu; Kexin Xu
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Paper Abstract

Glucose is one of the most important substances widely contained in organism and food, thus people pay much attention in researching and improving the way for the detection of glucose. Traditional ways, although precise and reliable when in high concentration and large amount of sample, have unconvincing performance in detecting mixture and solution with low concentration and micro-volume. As far as the ideal way is concerned, it should not only specifically detect the glucose and exclude other components in solution, but also meet the need of micro-sample (approximately 5μL) and low concentration. We introduced D-galactose/D-glucose Binding Protein (GGBP) - a kind of protein which has the ability to absorb the glucose specifically, to construct a novel surface plasmon resonance measuring system. By immobilizing GGBP onto the surface of the SPR sensor, we develop a new detecting system for glucose testing in mixed solution. The experimental result indicates that compared with 0.1g/L before immobilization of GGBP, the detecting limit or the resolution of glucose testing rises to 1mg/L after the immobilization, the system succeeds in distinguishing glucose from other components in mixture, which reveals a bright future to apply SPR in the minimally invasive diabetes testing and food quality control.

Paper Details

Date Published: 15 February 2007
PDF: 9 pages
Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 64450N (15 February 2007); doi: 10.1117/12.699678
Show Author Affiliations
Xian Huang, Tianjin Univ. (China)
Dachao Li, Tianjin Univ. (China)
Haixia Yu, Tianjin Univ. (China)
Fuxiang Huang, Tianjin Univ. (China)
Xiaotang Hu, Tianjin Univ. (China)
Kexin Xu, Tianjin Univ. (China)

Published in SPIE Proceedings Vol. 6445:
Optical Diagnostics and Sensing VII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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