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Proceedings Paper

Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy
Author(s): Wen Lo; Hsin-Yuan Tan; Yuh-Ling Chang; Yen Sun; Sung-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong
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Paper Abstract

The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating the structural alterations and the pattern of microbial spreading during corneal infectious process in an in vitro organ culture model. The autofluorescence spectrum derived from pathogens allows us to monitoring the pattern of microbial spreading within corneal lamellae. In addition, the destruction and regeneration of second harmonic generating collagen during infectious process can also be monitored in a non-invasive fashion. Therefore we propose that multiphoton microscopy may potentially be applied as an effective monitoring tool for corneal infection studies.

Paper Details

Date Published: 26 March 2007
PDF: 8 pages
Proc. SPIE 6426, Ophthalmic Technologies XVII, 64260Y (26 March 2007); doi: 10.1117/12.699468
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Institute of Medical Engineering (Taiwan)
Chang Gung Univ. (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Yuh-Ling Chang, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. and National Taiwan Univ. Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. and National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6426:
Ophthalmic Technologies XVII
Bruce E. Stuck; Fabrice Manns; Per G. Söderberg; Michael Belkin M.D.; Arthur Ho, Editor(s)

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