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Proceedings Paper

Second-harmonic generation polarization microscopy by rotation of excitation light
Author(s): Peter Tramyeon Fwu; Chen-Kuan Chou; Wei-Liang Chen; Chen-Yuan Dong
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Paper Abstract

When imaging anisotropic samples with a laser scanning optical microscope, the results are often affected by the polarization of the excitation light source. Quantifying the polarization dependence of biological fibrous material such as muscle and collagen allows us to gain molecular information at length scale below the resolution of optical microscopes. One problem associated with rotating the direction of linearly polarized excitation light for an epi-illuminated laser scanning microscope is due to the reflective properties of the main dichroic mirror. Depending on the direction of the incident polarization, the dichroic mirror can induce different amount of phase retardation, thus altering the desired output polarization. In this work, we theoretically determined the needed combination of wave plates and their angular positions to compensate for the effect of the dichroic mirror, thus achieving any arbitrary linear polarization angle for the excitation incident on sample.

Paper Details

Date Published: 12 February 2007
PDF: 5 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421C (12 February 2007); doi: 10.1117/12.699458
Show Author Affiliations
Peter Tramyeon Fwu, National Taiwan Univ. (Taiwan)
Chen-Kuan Chou, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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