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Proceedings Paper

Effect of solvents on the fluorescence spectra of bacterial luciferase
Author(s): Irina E. Sukovataya; Natalya A. Tyulkova; Elisaveta V. Kaykova
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Paper Abstract

Bacteria luciferases catalyze the oxidation reaction of the long-chain aliphatic aldehyde and reduced flavinmononucleotide involving molecular oxygen to a respective fatty acid emitting light quanta in the visible spectrum. Fluorescence emission of luciferases from Photobacterium leiognathi dissolved in organic solvent-water mixtures was investigated. Methanol, acetone, dimethyl sulfoxide and formamide were used as organic solvents. As the methanol and acetone concentration is increased the emission maximum peak is decrease. In contrast, with dimethyl sulfoxide and formamide addition induced a increasing of the emission maximum intensity. The values of wavelength maximum (λmax) at the addition of this solvent can shows the spectra shifted to the red by about 12 nm. These increasing in the fluorescence intensity and in the λmax may be due to luciferase denaturation, resulting from the more intensive contact of chromospheres of luciferase with the solvent. At all used concentrations of methanol, acetone and formamide the shape of the fluorescence spectra was not changed. These studies demonstrate that the luciferase tryptophan fluorescence is sensitive to changes of physical-chemical property of enzyme environment. A comparison of activation/inactivation and fluorescence spectra of luciferase in methanol or acetone solutions shows that the extent of inactivation is larger than the extent of fluorescence changes at the same methanol or acetone concentration.

Paper Details

Date Published: 24 July 2006
PDF: 7 pages
Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61631I (24 July 2006); doi: 10.1117/12.697352
Show Author Affiliations
Irina E. Sukovataya, Krasnoyarsk State Univ. (Russia)
lnstitute of Biophysics (Russia)
Natalya A. Tyulkova, lnstitute of Biophysics (Russia)
Elisaveta V. Kaykova, Krasnoyarsk State Univ. (Russia)

Published in SPIE Proceedings Vol. 6163:
Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII
Valery V. Tuchin, Editor(s)

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