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Proceedings Paper

Microarrays for the evaluation of cell-biomaterial surface interactions
Author(s): H. Thissen; G. Johnson; G. McFarland; B. C. H. Verbiest; T. Gengenbach; N. H. Voelcker
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Paper Abstract

The evaluation of cell-material surface interactions is important for the design of novel biomaterials which are used in a variety of biomedical applications. While traditional in vitro test methods have routinely used samples of relatively large size, microarrays representing different biomaterials offer many advantages, including high throughput and reduced sample handling. Here, we describe the simultaneous cell-based testing of matrices of polymeric biomaterials, arrayed on glass slides with a low cell-attachment background coating. Arrays were constructed using a microarray robot at 6 fold redundancy with solid pins having a diameter of 375 μm. Printed solutions contained at least one monomer, an initiator and a bifunctional crosslinker. After subsequent UV polymerisation, the arrays were washed and characterised by X-ray photoelectron spectroscopy. Cell culture experiments were carried out over 24 hours using HeLa cells. After labelling with CellTracker® Green for the final hour of incubation and subsequent fixation, the arrays were scanned. In addition, individual spots were also viewed by fluorescence microscopy. The evaluation of cell-surface interactions in high-throughput assays as demonstrated here is a key enabling technology for the effective development of future biomaterials.

Paper Details

Date Published: 9 January 2007
PDF: 9 pages
Proc. SPIE 6413, Smart Materials IV, 64130B (9 January 2007); doi: 10.1117/12.696030
Show Author Affiliations
H. Thissen, CSIRO Molecular and Health Technologies (Australia)
G. Johnson, CSIRO Molecular and Health Technologies (Australia)
G. McFarland, CSIRO Molecular and Health Technologies (Australia)
B. C. H. Verbiest, CSIRO Molecular and Health Technologies (Australia)
Eindhoven Univ. of Technology (Netherlands)
T. Gengenbach, CSIRO Molecular and Health Technologies (Australia)
N. H. Voelcker, CSIRO Molecular and Health Technologies (Australia)
Flinders Univ. (Australia)

Published in SPIE Proceedings Vol. 6413:
Smart Materials IV
Nicolas H. Voelcker, Editor(s)

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