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Proceedings Paper

Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening
Author(s): Dirk U. Näther; Roger Fenske; Reynald Hurteaux; Sandra Majno; S. Desmond Smith
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Paper Abstract

A new plate reader (Nanotaurus) has been developed by Edinburgh Instruments that has the principle design features of a confocal microscope and utilises the technique of Time Correlated Single Photon Counting for data acquisition. The advantages of Fluorescence Lifetime Measurements in the nanosecond time scale and analysis methods to recover lifetime parameters are discussed based on experimental data. First working assays using changes of lifetime parameters are presented that clearly demonstrate the advantages of the new instrument for biochemical assays and show strong promise for cell-based assays, by utilising the independence of lifetime parameters from sample volume and concentration.

Paper Details

Date Published: 3 November 2006
PDF: 14 pages
Proc. SPIE 6372, Advanced Photon Counting Techniques, 637208 (3 November 2006); doi: 10.1117/12.688419
Show Author Affiliations
Dirk U. Näther, Edinburgh Instruments Ltd. (United Kingdom)
Roger Fenske, Edinburgh Instruments Ltd. (United Kingdom)
Reynald Hurteaux, Edinburgh Instruments Ltd. (United Kingdom)
Sandra Majno, Edinburgh Instruments Ltd. (United Kingdom)
S. Desmond Smith, Edinburgh Instruments Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 6372:
Advanced Photon Counting Techniques
Wolfgang Becker, Editor(s)

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