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Proceedings Paper

Surface plasmon resonance sensors for detection of survival of motor neuron protein
Author(s): Margaret Mitropanopoulos; Becki Campanaro; Michael Malone; Jean-Francois Masson; Ronald A. Nieman; Christian L. Lorson; Karl S. Booksh
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Paper Abstract

Surface Plasmon Resonance (SPR) spectroscopy is a potentially valuable tool for measuring protein-protein interactions and protein levels in vitro and in vivo. Fiber-optic based SPR allows for rapid quantitative measurement of disease markers such as the truncated (exons 1-7) survival of motor neuron (SMN) protein. Unlike micro fluidics-based SPR systems, sample loss is eliminated in fiber-optic SPR and the small size of the fiber optic probes (400μm or smaller) facilitates the potential for use in vivo. Recombinant SMN protein overexpressed in E. Coli as well as native SMN from cultured HeLa cells has been successfully measured using fiber-optic SPR.

Paper Details

Date Published: 25 October 2006
PDF: 11 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638008 (25 October 2006); doi: 10.1117/12.686497
Show Author Affiliations
Margaret Mitropanopoulos, Arizona State Univ. (United States)
Becki Campanaro, Arizona State Univ. (United States)
Michael Malone, Arizona State Univ. (United States)
Jean-Francois Masson, Arizona State Univ. (United States)
Ronald A. Nieman, Arizona State Univ. (United States)
Christian L. Lorson, Arizona State Univ. (United States)
Karl S. Booksh, Arizona State Univ. (United States)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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