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Proceedings Paper

UV LED excited time-gated luminescence flow cytometry: concepts and experimental evaluation
Author(s): Dayong Jin; Russell Connally; Jim Piper
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Paper Abstract

This paper presents experimental and theoretical studies of time-gated discrimination of long-lived luminescence (lifetime: 1~2000μs) labelled target-organisms against non-target autofluorescence background (lifetime: <100 ns) in flow cytometry. A theoretical model of such a TGL flow cytometer is developed which takes account of flow speed, illumination and detection apertures, fluorescence label lifetime, and pulsed illumination and gated detection timing sequences. Ultraviolet LED and channel photomultiplier were found to be practical as pulsed excitation sources and gated detector for TGL flow cytometry. The prototype cytometer was constructed and optimized to operate at 6 k Hz repetition rate of TGL cycles consisting of 100 μs LED excitation and ~60 μs gated detection. The spatial counting efficiency was evaluated by enumerating 5.5 μm diameter europium microspheres resulting in a counting accuracy approaching 100%.

Paper Details

Date Published: 25 October 2006
PDF: 8 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800L (25 October 2006); doi: 10.1117/12.685076
Show Author Affiliations
Dayong Jin, Macquarie Univ. (Australia)
Russell Connally, Macquarie Univ. (Australia)
Jim Piper, Macquarie Univ. (Australia)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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