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Proceedings Paper

Combined laser trapping and small-angle x-ray scattering experiment for the study of liposome colloidal microparticles
Author(s): Dan Cojoc; Enrico Ferrari; Valeria Garbin; Enzo Di Fabrizio; Heinz Amenitsch; Michel Rappolt; Barbara Sartori; Christian Riekel; Manfred Burghammer
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Paper Abstract

We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF). The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.

Paper Details

Date Published: 11 September 2006
PDF: 6 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63261M (11 September 2006); doi: 10.1117/12.680643
Show Author Affiliations
Dan Cojoc, CNR-INFM, Lab. Nazionale TASC (Italy)
Enrico Ferrari, CNR-INFM, Lab. Nazionale TASC (Italy)
Valeria Garbin, CNR-INFM, Lab. Nazionale TASC (Italy)
Enzo Di Fabrizio, CNR-INFM, Lab. Nazionale TASC (Italy)
Heinz Amenitsch, Institute of Biophysics Research (Austria)
Michel Rappolt, Institute of Biophysics Research (Austria)
Barbara Sartori, Institute of Biophysics Research (Austria)
Christian Riekel, European Synchrotron Radiation Facility (France)
Manfred Burghammer, European Synchrotron Radiation Facility (France)

Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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