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Proceedings Paper

Interactive space-time controlled application of different stimuli for cells dynamics study
Author(s): F. Belloni; S. Monneret; D. Marguet
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Paper Abstract

The way a cell reacts to a stimulus has a strong local nature based on the internal structure of it. Therefore models which describe, with a certain degree of precision, cell behaviours in response to a deformation of it as a whole cannot be extrapolated to the local response process. Under these assumptions the regional approach in single cell assays is earning more and more interest as it provides a more detailed insight on cells dynamics processes in terms of their morphology, and hence a more accurate description of the implied molecular entities. In the last decade, the development of a wide variety of optical trapping techniques has provided us a versatile tool to explore this locality of cells responses enabling a true "regional approach" and deepening our knowledge in the field. We here propose an apparatus based on multiple holographic optical tweezers and micro-stereolithography which allows an interactive control of the spatial-temporal characteristics of a trap pattern and the simultaneous application of different stimuli. These agents are kept separated from one another and from the cells via several custom-designed reservoirs fabricated via a micro-stereolithographic technique. It is worth noting that the work is more intended to propose a methodology or tool for meaningful assays rather than a new technique. As a consequence most of the efforts are being drawn towards to the simplification of the work flow to allow the device to actually be exploited and provide valuable data, and no more be a simple lab experiment.

Paper Details

Date Published: 5 October 2006
PDF: 10 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260R (5 October 2006); doi: 10.1117/12.680621
Show Author Affiliations
F. Belloni, CIML CNRS-INSERM (France)
Domaine Univ. de St. Gerome (France)
S. Monneret, Domaine Univ. de St. Gerome (France)
D. Marguet, CIML CNRS-INSERM (France)

Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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