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Proceedings Paper

SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement
Author(s): K. Yoshiki; M. Hashimoto; T. Araki M.D.
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Paper Abstract

We have developed a second-harmonic-generation (SGH) microscope to observe the three-dimensional molecular orientation with three-dimensional high spatial resolution using a polarization mode converter. The mode converter consists of a parallel-aligned nematic-liquid-crystal spatial-light-modulator (PAL-SLM) and quarter-waveplates, and converts a incident linearly polarized beam to orthogonal linearly polarized beams or radially polarized beam. We combined the mode converter with SHG microscope to obtain the local information of the three-dimensional molecular orientation. We demonstrated the detection of three-dimensional molecular orientation of collagen fiber in human Achilles' tendon. For high precision three-dimensional molecular orientation measurement, we propose a technique to calibrate the dependence of SHG detection efficiencies on molecular orientation using a liposome.

Paper Details

Date Published: 9 September 2006
PDF: 8 pages
Proc. SPIE 6290, Laser Beam Shaping VII, 62900F (9 September 2006); doi: 10.1117/12.680506
Show Author Affiliations
K. Yoshiki, Osaka Univ. (Japan)
M. Hashimoto, Osaka Univ. (Japan)
T. Araki M.D., Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 6290:
Laser Beam Shaping VII
Fred M. Dickey; David L. Shealy, Editor(s)

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