
Proceedings Paper
Three-dimensional assessment of brain tissue morphologyFormat | Member Price | Non-Member Price |
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Paper Abstract
The microstructure of brain tissues becomes visible using different types of optical microscopy after the tissue sectioning. This
preparation procedure introduces stress and strain in the anisotropic and inhomogeneous soft tissue slices, which are several
10 μm thick. Consequently, the three-dimensional dataset, generated out of the two-dimensional images with lateral submicrometer
resolution, needs algorithms to correct the deformations, which can be significant for mellow tissue such as brain
segments. The spatial resolution perpendicular to the slices is much worse with respect to the lateral sub-micrometer
resolution. Therefore, we propose as complementary method the synchrotron-radiation-based micro computed tomography
(SRμCT), which avoids any kind of preparation artifacts due to sectioning and histological processing and yields true
micrometer resolution in the three orthogonal directions. The visualization of soft matter by the use of SRμCT, however, is
often based on elaborate staining protocols, since the tissue exhibits (almost) the same x-ray absorption as the surrounding
medium. Therefore, it is unexpected that human tissue from the pons and the medulla oblongata in phosphate buffer show
several features such as the blood vessels and the inferior olivary nucleus without staining. The value of these tomograms lies
especially in the precise non-rigid registration of the different sets of histological slices. Applications of this method to larger
pieces of brain tissue, such as the human thalamus are planned in the context of stereotactic functional neurosurgery.
Paper Details
Date Published: 7 September 2006
PDF: 8 pages
Proc. SPIE 6318, Developments in X-Ray Tomography V, 631803 (7 September 2006); doi: 10.1117/12.680312
Published in SPIE Proceedings Vol. 6318:
Developments in X-Ray Tomography V
Ulrich Bonse, Editor(s)
PDF: 8 pages
Proc. SPIE 6318, Developments in X-Ray Tomography V, 631803 (7 September 2006); doi: 10.1117/12.680312
Show Author Affiliations
Bert Müller, ETH Zürich (Switzerland)
Marco Germann, ETH Zürich (Switzerland)
Univ. Hospital Zurich (Switzerland)
Marco Germann, ETH Zürich (Switzerland)
Univ. Hospital Zurich (Switzerland)
Daniel Jeanmonod, Univ. Hospital Zürich (Switzerland)
Anne Morel, Univ. Hospital Zürich (Switzerland)
Anne Morel, Univ. Hospital Zürich (Switzerland)
Published in SPIE Proceedings Vol. 6318:
Developments in X-Ray Tomography V
Ulrich Bonse, Editor(s)
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