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Proceedings Paper

Optically controlled manipulation of live cells using optoelectronic tweezers
Author(s): Aaron T. Ohta; Pei-Yu Chiou; Ming C. Wu
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Paper Abstract

Optoelectronic tweezers (OET) provides a non-invasive, low-power, optical manipulation tool for trapping, transporting, and separating microparticles, cells, and other bioparticles. The OET device uses a photosensitive layer to form "virtual electrodes" upon exposure to light, creating non-uniformities in an applied electric field. The electric field gives rise to a force known as dielectrophoresis: microparticles move as a result of the non-uniformities in the electric field imparting unequal forces on the induced dipoles of the particles. These virtual electrodes can be actuated with low optical intensities, enabling the use of incoherent light sources and direct imaging techniques to create optical manipulation patterns in real-time. In this paper, we demonstrate OET operation on live cells, including the trapping and manipulation of red and white blood cells, and the automated collection of HeLa cells. Automated size-based sorting is performed on a mixture of 15- and 20-μm-diameter polystyrene beads, and dielectric property-based separation is used to differentiate between live and dead white blood cells.

Paper Details

Date Published: 5 October 2006
PDF: 11 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 632617 (5 October 2006); doi: 10.1117/12.679710
Show Author Affiliations
Aaron T. Ohta, Univ. of California, Berkeley (United States)
Pei-Yu Chiou, Univ. of California, Berkeley (United States)
Ming C. Wu, Univ. of California, Berkeley (United States)

Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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