Share Email Print

Proceedings Paper

Assessment of murine bone ultrastructure using synchrotron light: towards nano-computed tomography
Author(s): Philipp Schneider; Romain Voide; Martin Stauber; Marco Stampanoni; Leah Rae Donahue; Peter Wyss; Urs Sennhauser; Ralph Müller
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

To describe the different aspects of bone quality, we follow a hierarchical approach and assess bone tissue properties in different regimes of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes we developed different synchrotron radiation (SR) based computed-tomography (CT) methods to assess murine bone ultrastructure. In a first step, a tubular system and the osteocyte lacunar system within murine cortical bone have been established as novel ultrastructural quantitative traits. Results in two mouse strains showed that morphometry of these quantitative traits was dependent on strain and partially on gender, and that their scaling behavior with bone size was fundamentally different. In a second step, we explored bone competence on an ultrastructural level and related our findings to the two ultrastructural quantitative traits introduced before. We showed that SR CT imaging is a powerful tool to investigate the initiation and propagation of microcracks, which may alter bone quality and may lead to increased fracture risk by means of microdamage accumulation. In summary, investigation of ultrastructural bone tissue properties will eventually lead to a better understanding of bone quality and its relative contribution to bone competence.

Paper Details

Date Published: 7 September 2006
PDF: 9 pages
Proc. SPIE 6318, Developments in X-Ray Tomography V, 63180C (7 September 2006); doi: 10.1117/12.679427
Show Author Affiliations
Philipp Schneider, ETH Zürich (Switzerland)
Romain Voide, ETH Zürich (Switzerland)
Martin Stauber, ETH Zürich (Switzerland)
Marco Stampanoni, Paul Scherrer Institut (Switzerland)
Leah Rae Donahue, The Jackson Lab. (United States)
Peter Wyss, Swiss Federal Labs. for Material Testing and Research (Switzerland)
Urs Sennhauser, Swiss Federal Labs. for Material Testing and Research (Switzerland)
Ralph Müller, ETH Zürich (Switzerland)

Published in SPIE Proceedings Vol. 6318:
Developments in X-Ray Tomography V
Ulrich Bonse, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?