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Proceedings Paper

Multi-photon microscopy of tobacco-exposed organotypic skin models
Author(s): Belinda Dao; Alissa Yamazaki; Chung Ho Sun; Zifu Wang; Nguyen Pham; Michael Oldham; Brian J. F. Wong M.D.
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Paper Abstract

Cigarette smoking is the most preventable cause of death in the United States. Researchers have extensively studied smoking in regards to its association with cancer, cardiovascular, and pulmonary disease. In contrast, the impact of cigarette smoking on skin has received much less attention. To provide a better understanding of the effect of cigarette smoking on the human dermal layer, this study used multi-photon microscopy (MPM) to examine collagen in organotypic skin models exposed to cigarette smoke condensate (CSC). Adult and neonatal organotypic tissue-engineered artificial skin models (RAFTs) were constructed and exposed to varying concentrations of CSC. Imaging of the RAFTs was performed using MPM and second-harmonic generation signals (SHG), which allowed for collagen structure to be viewed and analyzed as well as for collagen density to be assessed from derived depth-dependent decay (DDD) values. RAFT contraction as related to exposure concentration was monitored as well. Results indicated a dose dependent between contraction rates and CSC concentration. Collagen structure showed more preservation of its original structure at a greater depth in RAFTs with higher concentrations of CSC. No clear trends could be drawn from analysis of derived DDD values.

Paper Details

Date Published: 22 February 2006
PDF: 12 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60781V (22 February 2006); doi: 10.1117/12.674185
Show Author Affiliations
Belinda Dao, Beckman Laser Institute (United States)
Alissa Yamazaki, Beckman Laser Institute (United States)
Chung Ho Sun, Beckman Laser Institute (United States)
Zifu Wang, Univ. of California, Irvine (United States)
Nguyen Pham, Univ. of California, Irvine (United States)
Michael Oldham, Univ. of California, Irvine (United States)
Brian J. F. Wong M.D., Beckman Laser Institute (United States)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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