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Proceedings Paper

A study of interaction between FITC- labeled antibody and optical fiber surface
Author(s): K. F. Chao; Y. L. Zhang; X. G. Kong; Q. H. Zeng; R. L. Liu; X. Wang; L. Y. Feng; Y. J. Sun
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Paper Abstract

The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper, FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore, red shift of the FITC fluorescence dye emission peak was observed on different fiber surface, exhibiting different interaction between FITC- labeled antibody and surface. Finally, under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.

Paper Details

Date Published: 24 January 2006
PDF: 8 pages
Proc. SPIE 6029, ICO20: Materials and Nanostructures, 602922 (24 January 2006); doi: 10.1117/12.667842
Show Author Affiliations
K. F. Chao, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
Chinese Academy of Sciences (China)
Y. L. Zhang, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
X. G. Kong, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
Q. H. Zeng, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
R. L. Liu, Jinlin Univ. (China)
X. Wang, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
Chinese Academy of Sciences (China)
L. Y. Feng, ChangChun Institute of Optics, Fine Mechanics and Physics (China)
Chinese Academy of Sciences (China)
Y. J. Sun, Jinlin Univ. (China)


Published in SPIE Proceedings Vol. 6029:
ICO20: Materials and Nanostructures
Wei Lu; Jeff Young, Editor(s)

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