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Proceedings Paper

Three-dimensional imaging and recognition of microorganisms using computational holography
Author(s): Bahram Javidi; Seokwon Yeom; Inkyu Moon; Edward Carapezza
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Paper Abstract

In this keynote address, we introduce three-dimensional (3D) sensing, visualization and recognition of microorganisms using microscopy-based single-exposure on-line (SEOL) digital holography. A coherent Mach-Zehnder interferometer records Fresnel diffraction field by a single on-line exposure to generate a microscopic digital hologram. Complex amplitude distribution is numerically reconstructed by the inverse Fresnel transform at arbitrary depth planes. After the reconstruction of volumetric complex images, 3D biological micro-objects are segmented and features are extracted by Gabor-based wavelets. The graph matching technique searches predefined 3D morphological shapes of reference biological microorganisms. Preliminary experimental results using sphacelaria alga and tribonema aequale alga are presented.

Paper Details

Date Published: 19 May 2006
PDF: 8 pages
Proc. SPIE 6234, Automatic Target Recognition XVI, 623405 (19 May 2006); doi: 10.1117/12.664209
Show Author Affiliations
Bahram Javidi, Univ. of Connecticut (United States)
Seokwon Yeom, Univ. of Connecticut (United States)
Inkyu Moon, Univ. of Connecticut (United States)
Edward Carapezza, Defense Advanced Research Projects Agency (United States)

Published in SPIE Proceedings Vol. 6234:
Automatic Target Recognition XVI
Firooz A. Sadjadi, Editor(s)

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