Share Email Print
cover

Proceedings Paper

Micrometer sized immobilization of protein molecules onto quartz, silicium and gold.
Author(s): Steffen B. Petersen; Maria Teresa Neves-Petersen; Søren Klitgaard; Meg Crookshanks Duroux
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

We demonstrate that ultraviolet light can be used to make sterically oriented covalent immobilization of a large variety of protein molecules onto either gold or thiolated quartz or silicium. The reaction mechanism behind the reported new technology involves light induced breakage of disulphide bridges in proteins upon UV illumination of nearby aromatic amino acids, resulting in the formation of free, reactive thiol groups that will form covalent bonds with thiol reactive surfaces. The protein molecules in general retain their function. The size of the immobilization spot is determined by the dimension of the UV beam. In principle, the spot size may be as small as 1 micrometer or less. We have developed the necessary technology for preparing large protein arrays of enzymes and fragments of monoclonal antibodies. Dedicated Image Processing Software has been developed for making quality assessment of the protein arrays. A multitude of important application areas such as drug carriers and drug delivery, bioelectronics, carbon nanotubes, nanoparticles as well as protein glue are discussed.

Paper Details

Date Published: 13 February 2006
PDF: 12 pages
Proc. SPIE 6106, Photon Processing in Microelectronics and Photonics V, 61061Y (13 February 2006); doi: 10.1117/12.664086
Show Author Affiliations
Steffen B. Petersen, Aalborg Univ. (Denmark)
Maria Teresa Neves-Petersen, Aalborg Univ. (Denmark)
Søren Klitgaard, Aalborg Univ. (Denmark)
Meg Crookshanks Duroux, Aalborg Univ. (Denmark)


Published in SPIE Proceedings Vol. 6106:
Photon Processing in Microelectronics and Photonics V
David B. Geohegan; Tatsuo Okada; Craig B. Arnold; Frank Träger; Jan J. Dubowski; Michel Meunier; Andrew S. Holmes, Editor(s)

© SPIE. Terms of Use
Back to Top