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Proceedings Paper

Applications of quantum dots in cell biology
Author(s): Margarida Barroso; Roshanak Mehdibeigi; Louise Brogan
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Paper Abstract

Quantum dots promise to revolutionize the way fluorescence imaging is used in the Cell Biology field. The unique fluorescent spectral characteristics, high photostability, low photobleaching and tight emission spectra of quantum dots, position them above traditional dyes. Here we will address the ability of EviTags, which are water stabilized quantum dot products from Evident Technologies, to behave as effective FRET donors in cells. EviTag-Hops Yellow (HY; Emission 566nm; Donor) conjugated to biotin were bound to stretapvidin-Alexa568 (Acceptor) conjugates. These HYbiotin-streptavidin-Alexa568 FRET EviTag conjugates were then internalized by fluid-phase into non-polarized MDCK cells. Confocal microscopy detects these FRET EviTag conjugates in endocytic compartments, suggesting that EviTags can be used to track fluid-phase internalization and trafficking. EviTags are shown here to be effective FRET donors when internalized into cells. Upon pairing with the appropriate acceptor dyes, quantum dots will reduce the laborious data processing that is required to compensate for bleed through contamination between organic dye donor and acceptor pair signals. The EviTag technology will simplify and expand the use of FRET in the analysis of cellular processes that may involve protein-protein interactions and other complex cellular processes.

Paper Details

Date Published: 27 March 2006
PDF: 9 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960R (27 March 2006); doi: 10.1117/12.663336
Show Author Affiliations
Margarida Barroso, Albany Medical College (United States)
Roshanak Mehdibeigi, Albany Medical College (United States)
Louise Brogan, Evident Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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