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Proceedings Paper

Multiplexed and Quantitative Study of Biomarker Expression in Tumor Specimens Using Quantum Dots
Author(s): Aileen Wu; Lawrence True; Xiaohu Gao
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Paper Abstract

When conjugated with targeting molecules, quantum dots (QD) can be used as powerful cancer diagnostic tools providing the molecular profiles of cancer cases based on common clinical biopsies. Such personalized analyses will enable doctors to treat and manage the patients' diseases more effectively. The unique optical properties (e.g., size-tunable emission, simultaneous excitation, high brightness and photostability) of these nanoparticles make them superior to conventionally popular organic fluorophores1-2. Polymer-encapsulated, antibody-tagged QDs were prepared and used to successfully stain both fixed and live cells as well as clinical formalin-fixed paraffin-embedded (FFPE) tissue sections. In the tissue staining study, QD bioconjugates targeting mutated p53 and early growth response protein (egr-1) were used to examine prostate cancer tissues. The tissue slides were then analyzed with a wavelength-resolved spectrometer to accurately quantify the protein expression levels. In comparison to traditional qualitatively based diagnostic procedures, quantum dot nanotechnology allows for a more quantitative, rigorous and objective analysis of tissue specimens in question. In addition, new developments in imaging instrumentation could automate spectroscopy measurements and data analysis.

Paper Details

Date Published: 27 March 2006
PDF: 9 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60961C (27 March 2006); doi: 10.1117/12.661783
Show Author Affiliations
Aileen Wu, Univ. of Washington (United States)
Lawrence True, Univ. of Washington Medical Ctr. (United States)
Xiaohu Gao, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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