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Proceedings Paper

Injection and manipulation of silicon microbeads in a customized microfluidic platform
Author(s): Daniel Hoffmann; Des Brennan; Michael Loughran
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Paper Abstract

The first on-chip injection and manipulation of optically encoded, silicon microbeads in a microfluidic platform is reported. Encoded microbeads of different shapes and sizes were fabricated in silicon via standard microfabrication techniques. The optical signature consisted of a series of lithographically defined bar-codes, which can be identified by a laser detection system. In-situ identification of encoded microbeads was possible at microbead velocities ≤ 50 cm per second. The microbeads can also be transported within a channel network in accordance with the encoded optical signature of each bead. The microbead transport is controlled by the laminar flow of a liquid in pressure driven microchannels. Hydrodynamic pulsing facilitated single and multiple injection of microbeads from a reservoir into the laminar fluid stream of a branched microfluidic network. Careful control of the fluid velocity and alteration of the microchannel geometry also enabled manipulation of microbead velocity. The incorporation of five pillars to retain microbeads at a specific location within the microchannel network formed the basis of a reaction chamber for on chip functionalization of microbeads. The principle of hydrodynamic switching was utlized to re-direct the transport of microbeads at a branched microfluidic network. In the final part of this research we verify that this microbead technology is suitable for detection of specific target DNA.

Paper Details

Date Published: 23 January 2006
PDF: 8 pages
Proc. SPIE 6112, Microfluidics, BioMEMS, and Medical Microsystems IV, 61120E (23 January 2006); doi: 10.1117/12.660342
Show Author Affiliations
Daniel Hoffmann, Univ. College Cork (Ireland)
Des Brennan, Univ. College Cork (Ireland)
Michael Loughran, Univ. College Cork (Ireland)


Published in SPIE Proceedings Vol. 6112:
Microfluidics, BioMEMS, and Medical Microsystems IV
Ian Papautsky; Wanjun Wang, Editor(s)

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