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Influence of low level laser radiation on migration of stem cells
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Paper Abstract

The long-term effects of low level laser therapy can involve mechanisms connected with activation of migration of stem cells towards damaged areas. Stromal cell-derived factor-1 alpha (SDF-1) plays a critical role in stem cell migration towards areas of tissue injury and hypoxia. This study examines the influence of laser light on migration of stem cells (FDCP-mix stem cell line) in absence and in presence of SDF-1, using Transwell system with 2 connected chambers separated by a filter. The first chamber contained medium and stem cells, second chamber contained medium with or without SDF. Group 1 cells served as a control. Cells of groups 2 and 5 were irradiated by red diode laser (660 nm, 20 mW, 15 min). Cells of groups 3 and 6 received infrared (IR) diode laser (958 nm, 36 mW, 15 min). Group 4 was SDF-1 control. Stem cells were seeded into first chamber of the Transwell system, and filters were placed into the wells containing medium with 150 ng/ml SDF-1 (groups 4,5,6) or without SDF-1 (groups 1,2,3). After incubation cells were collected and counted. The count of spontaneously migrated cells was 1496,5±409 (100%) in case of control. Red and IR laser light increased motility of stem cells up to 1892±283 (126%) and 2255,5±510 (151%) accordingly. Influence of SDF-1 caused directed migration of 3365,5±489 cells (225%). Combined effects of light irradiation and SDF-1 were significantly stronger (p<0,05): 5813±1199 (388%) for SDF-1 and red laser , 6391,5±540 (427%) for SDF-1 and IR laser. Study shows that laser radiation can up-regulate stem cell migration towards higher SDF-1 gradient.

Paper Details

Date Published: 25 October 2005
PDF: 6 pages
Proc. SPIE 5968, Laser Florence 2004: A Window on the Laser Medicine World, 596808 (25 October 2005); doi: 10.1117/12.660042
Show Author Affiliations
Levon V. Gasparyan M.D., EMRED Oy (Finland)
Grigory Brill, Saratov State Medical Univ. (Russia)
Anu M. Makela M.D., ABER Institute (Finland)

Published in SPIE Proceedings Vol. 5968:
Laser Florence 2004: A Window on the Laser Medicine World
Leonardo Longo; Khalil A. Khatri; Mihail-Lucian Pascu; Wilhelm R.A. Waidelich, Editor(s)

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