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Proceedings Paper

Elimination of leukemic cells from human transplants by laser nano-thermolysis
Author(s): Dmitri Lapotko; Ekaterina Lukianova; Michail Potapnev; Olga Aleinikova; Alexander Oraevsky
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Paper Abstract

We describe novel ex vivo method for elimination of tumor cells from bone marrow and blood, Laser Activated Nano-Thermolysis for Cell Elimination Technology (LANTCET) and propose this method for purging of transplants during treatment of leukemia. Human leukemic cells derived from real patients with different diagnoses (acute lymphoblastic leukemias) were selectively damaged by LANTCET in the experiments by laser-induced micro-bubbles that emerge inside individual specifically-targeted cells around the clusters of light-absorbing gold nanoparticles. Pretreatment of the transplants with diagnosis-specific primary monoclonal antibodies and gold nano-particles allowed the formation of nanoparticle clusters inside leukemic cells only. Electron microscopy found the nanoparticulate clusters inside the cells. Total (99.9%) elimination of leukemic cells targeted with specific antibodies and nanoparticles was achieved with single 10-ns laser pulses with optical fluence of 0.2 - 1.0 J/cm2 at the wavelength of 532 nm without significant damage to normal bone marrow cells in the same transplant. All cells were studied for the damage/viability with several control methods after their irradiation by laser pulses. Presented results have proved potential applicability of developed LANTCET technology for efficient and safe purging (cleaning of residual tumor cells) of human bone marrow and blood transplants. Design of extra-corporeal system was proposed that can process the transplant for one patient for less than an hour with parallel detection and counting residual leukemic cells.

Paper Details

Date Published: 6 March 2006
PDF: 8 pages
Proc. SPIE 6086, Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 60860I (6 March 2006); doi: 10.1117/12.658139
Show Author Affiliations
Dmitri Lapotko, Luikov Heat and Mass Transfer Institute (Belarus)
Ekaterina Lukianova, Luikov Heat and Mass Transfer Institute (Belarus)
Michail Potapnev, Belarusian Research and Production Ctr. for Hematology and Transfusiology (Belarus)
Olga Aleinikova, Belarusian Research Ctr. for Pediatric Hematology and Oncology (Belarus)
Alexander Oraevsky, Fairway Medical Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 6086:
Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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