The oxidative stress initiated by photodynamic therapy (PDT) results in accumulation of ceramide in various cell types undergoing apoptosis. Apoptotic responses to PDT are similar to those of the oxidative stress inducer and the Bcl-2 inhibitor HA 14-1 in murine leukemia L1210 cells. Here we tested whether de novo ceramide accumulation can be induced by HA 14-1 during apoptosis in Jurkat T human leukemia cells. Similar to photosensitization with Pc 4, treatment of Jurkat cells with HA14-1 leads to DEVDase activation and nuclear apoptosis. However, unlike Pc 4-PDT, HA 14-1 did not induce accumulation of de novo ceramide. The data suggest that, compared to HA 14-1, de novo ceramide accumulation is a distinct response to PDT in Jurkat cells.

Date Published: 6 March 2006
PDF: 8 pages
Proc. SPIE 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV, 61390E (6 March 2006); doi: 10.1117/12.657692

Published in SPIE Proceedings Vol. 6139:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV
David Kessel, Editor(s)