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Proceedings Paper

Reproducibility of 3D micro-CT gray-scale and structural dimension data in longitudinal studies
Author(s): Steven M. Jorgensen; Diane R. Eaker; Andrew J. Vercnocke; Erik L. Ritman M.D.
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Paper Abstract

Repeated micro-CT scanning of a number of iliac crest biopsies enabled us to quantitate the variation in CT image gray-scale and spatial geometry due to variables such as specimen orientation, projection magnification, voxel size and slight differences in x-ray photon energy in each of the different scans. Using the micro-CT scanner on beamline X2B at the Brookhaven National Laboratory's National Synchrotron Light Source, we rescanned several iliac crest bone biopsy specimens, and a test phantom made of calcium hydroxyapatite, at repeated scanning sessions and evaluated the reproducibility of the spatial geometry and gray-scale haracteristics of the specimens. This scanner consists of a Bragg diffiaction source of monochromatic x-rays, a computer controlled high precision specimen rotation and translation stage assembly, and a fluorescent crystal and CCD array system for imaging the specimen at each of the angles of view around its axis of rotation during the scanning sequence. The 3-D micro-CT images consisted of up to 1024x24002, 4 μm, cubic voxels, each with 16-bit gray-scale. We also reconstructed the images at 16,32 and 48 μm voxel resolution. Partial volume effects at the surface of the bone were diminished by 'eroding' the surface voxels in the 4 μm images, but significantly changed the outcome at greater voxel size. Reproducibility of the mineral content of bone, at mean bone opacity value, was ± 28.8 mg/cm3, i.e., 2.56%, in a 4 μm cubic voxel at the 95% confidence level.

Paper Details

Date Published: 13 March 2006
PDF: 9 pages
Proc. SPIE 6143, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, 61433H (13 March 2006); doi: 10.1117/12.654436
Show Author Affiliations
Steven M. Jorgensen, Mayo Clinic College of Medicine (United States)
Diane R. Eaker, Mayo Clinic College of Medicine (United States)
Andrew J. Vercnocke, Mayo Clinic College of Medicine (United States)
Erik L. Ritman M.D., Mayo Clinic College of Medicine (United States)

Published in SPIE Proceedings Vol. 6143:
Medical Imaging 2006: Physiology, Function, and Structure from Medical Images
Armando Manduca; Amir A. Amini, Editor(s)

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