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Proceedings Paper

Fluorescence guided resection for malignant glioma using 5-aminolevulinic acid
Author(s): Herbert Stepp; Tobias Beck; Thomas Pongratz; Thomas Meinel; Jörg Ch. Tonn; Walter Stummer
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Paper Abstract

Background: Oral application of 20 mg/kg body weight of 5-aminolevulinic acid (ALA) results in a highly specific accumulation of fluorescent Protoporphyrin IX (PpIX) in malignant glioma tissue. In a clinical phase II study, Photodynamic Therapy (PDT) to postsurgical tumor-remnants was monitored by fluorescence spectroscopy and photobleaching of PpIX was detected. Methods: Fluorescence spectra were measured from the tumor remnant pre and at intervals during PDT with 100, 150 and 200 J/cm2 and from adjacent normal cortex. The spectra obtained were fitted with spectra from pure PpIX, autofluorescence and photoproducts, the fit-parameters quantifying the contributions of these components to the measured spectrum. Results: The PpIX-fluorescence bleached to 8%, 16% and 1% of the initial intensity for the 100, 150 and 200 J/cm2-groups (median values). Photoproduct formation was minimal. PpIX-fluorescence of normal cortex was 6% of tumor remnant fluorescence (median, range: 0% to 49%).

Paper Details

Date Published: 22 February 2006
PDF: 7 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60782W (22 February 2006); doi: 10.1117/12.652069
Show Author Affiliations
Herbert Stepp, Univ. Clinic Munich (Germany)
Tobias Beck, Univ. Clinic Munich (Germany)
Thomas Pongratz, Univ. Clinic Munich (Germany)
Thomas Meinel, J.-W. Goethe Univ. Frankfurt (Germany)
Jörg Ch. Tonn, Univ. Clinic Munich (Germany)
Walter Stummer, Univ. Clinic Düsseldorf (Germany)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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