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Proceedings Paper

Combined time- and depth-resolved autofluorescence spectroscopy for tissue diagnosis
Author(s): Yicong Wu; Jianan Y. Qu
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Paper Abstract

A fluorescence spectroscopy system combining depth- and time-resolved measurements is developed to investigate the layered fluorescence temporal characteristics of epithelial tissue. It is found that esophageal tissue structure can be resolved well by means of the autofluorescence time-resolved decay process with 375-, 405- and 435- nm excitation. The decay of the autofluorescence signals can be accurately fitted with a dual-exponential function consisting of a short lifetime (0.4 ~ 0.6 ns) and a long lifetime (3 ~ 4 ns) components. The short lifetime component dominates the decay of normal epithelial fluorescence while the decay of the signals from keratinized epithelium and stroma are mainly determined by the long lifetime component. The ratio of the amplitudes of two components provides the information of fine structure of epithelial tissue. This study demonstrates that the combined depth- and time-resolved measurements can potentially provide accurate information for the diagnosis of tissue pathology.

Paper Details

Date Published: 25 February 2006
PDF: 5 pages
Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 608018 (25 February 2006); doi: 10.1117/12.651391
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)

Published in SPIE Proceedings Vol. 6080:
Advanced Biomedical and Clinical Diagnostic Systems IV
Gerald E. Cohn; Warren S. Grundfest M.D.; David A. Benaron M.D.; Tuan Vo-Dinh, Editor(s)

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