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Proceedings Paper

Bacteria-clay interactions investigated by light scattering and phase contrast microscopy
Author(s): Alexandra Alimova; Karin Block; Elizabeth Rudolph; A. Katz; J. C. Steiner; P. Gottlieb; R. R. Alfano
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Paper Abstract

Light scattering experiments and phase contrast microscopy are used to evaluate the aggregate-forming characteristics of simple clay-bacteria mixtures. Colloidal suspensions of negatively charged Pseudomonas syringae (Ps) and Mg2+-, Li+ - or Ca2+ -exchanged smectite (and non-exchanged smectite) are flocculated in neutral (pH 7) aqueous media. Aggregate formation is monitored using changes in optical transmission. Clustering is observed in all the clay-bacteria preparations. The Li+-substituted clay aggregates average 50-300 microns in diameter, in contrast to the Ca2+- substituted clay that produces aggregates of 10-50 microns in diameter. Light scattering measurements indicate that aggregates begin forming 3 hours after mixing and that the (larger sized) aggregates exhibit less scattering than a mixture with an equivalent concentration of unattached Ps and clay particles.

Paper Details

Date Published: 27 February 2006
PDF: 4 pages
Proc. SPIE 6094, Optical Diagnostics and Sensing VI, 60940E (27 February 2006); doi: 10.1117/12.648042
Show Author Affiliations
Alexandra Alimova, City College, CUNY (United States)
Karin Block, City College, CUNY (United States)
Elizabeth Rudolph, City College, CUNY (United States)
A. Katz, City College, CUNY (United States)
J. C. Steiner, City College, CUNY (United States)
P. Gottlieb, City College, CUNY (United States)
R. R. Alfano, City College, CUNY (United States)

Published in SPIE Proceedings Vol. 6094:
Optical Diagnostics and Sensing VI
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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