Share Email Print

Proceedings Paper

3D-localization of the a-subunit in F0F1-ATP synthase by time resolved single-molecule FRET
Author(s): Monika G. Düser; Nawid Zarrabi; Yumin Bi; Boris Zimmermann; Stanley D. Dunn; Michael Börsch
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

FoF1-ATP synthases catalyze the ATP formation from ADP and phosphate in the membranes of mitochondria, chloroplasts and bacteria. Internal rotation of subunits couples the chemical reaction at the F1 part to the proton translocation through the Fo part. In these enzymes, the membrane-embedded a-subunit is part of the non-rotating 'stator' subunits and provides the proton channel of the Fo motor. At present, the relative position of the a-subunit is not known. We examined the rotary movements of the ε-subunit with respect to the non-rotating a-subunit by time resolved singlemolecule fluorescence resonance energy transfer (FRET) using a novel pulsed laser diode. Rotation of the ε-subunit during ATP hydrolysis was divided into three major steps. The stopping positions of ε resulted in three distinct FRET efficiency levels and FRET donor lifetimes. From these FRET efficiencies the position of the FRET donor at the asubunit was calculated. Different populations of the three resting positions of ε, which were observed previously, enabled us to scrutinize the models for the position of the a-subunit in the Fo part.

Paper Details

Date Published: 27 February 2006
PDF: 16 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920H (27 February 2006); doi: 10.1117/12.647988
Show Author Affiliations
Monika G. Düser, Univ. Stuttgart (Germany)
Nawid Zarrabi, Univ. Stuttgart (Germany)
Yumin Bi, Univ. of Western Ontario (Canada)
Boris Zimmermann, Albert-Ludwig-Univ. Freiburg (Germany)
Stanley D. Dunn, Univ. of Western Ontario (Canada)
Michael Börsch, Univ. Stuttgart (Germany)

Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?