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Proceedings Paper

Detection of high-risk atherosclerotic lesions by time-resolved fluorescence spectroscopy based on the Laguerre deconvolution technique
Author(s): Javier A. Jo; Qiyin Fang; Thanassis Papaioannou; Jianhua Qiao; M. C. Fishbein M.D.; B. Beseth; A. H. Dorafshar; T. Reil; D. Baker M.D.; J. Freischlag; L. Marcu
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Paper Abstract

This study introduces new methods of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) data analysis for tissue characterization. These analytical methods were applied for the detection of atherosclerotic vulnerable plaques. Upon pulsed nitrogen laser (337 nm, 1 ns) excitation, TR-LIFS measurements were obtained from carotid atherosclerotic plaque specimens (57 endarteroctomy patients) at 492 distinct areas. The emission was both spectrally- (360-600 nm range at 5 nm interval) and temporally- (0.3 ns resolution) resolved using a prototype clinically compatible fiber-optic catheter TR-LIFS apparatus. The TR-LIFS measurements were subsequently analyzed using a standard multiexponential deconvolution and a recently introduced Laguerre deconvolution technique. Based on their histopathology, the lesions were classified as early (thin intima), fibrotic (collagen-rich intima), and high-risk (thin cap over necrotic core and/or inflamed intima). Stepwise linear discriminant analysis (SLDA) was applied for lesion classification. Normalized spectral intensity values and Laguerre expansion coefficients (LEC) at discrete emission wavelengths (390, 450, 500 and 550 nm) were used as features for classification. The Laguerre based SLDA classifier provided discrimination of high-risk lesions with high sensitivity (SE>81%) and specificity (SP>95%). Based on these findings, we believe that TR-LIFS information derived from the Laguerre expansion coefficients can provide a valuable additional dimension for the diagnosis of high-risk vulnerable atherosclerotic plaques.

Paper Details

Date Published: 22 February 2006
PDF: 9 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60782H (22 February 2006); doi: 10.1117/12.647560
Show Author Affiliations
Javier A. Jo, Cedars-Sinai Medical Ctr. (United States)
Qiyin Fang, Cedars-Sinai Medical Ctr. (United States)
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Jianhua Qiao, Univ. of California/Los Angeles (United States)
M. C. Fishbein M.D., David Geffen-UCLA School of Medicine (United States)
B. Beseth, David Geffen-UCLA School of Medicine (United States)
A. H. Dorafshar, David Geffen-UCLA School of Medicine (United States)
T. Reil, David Geffen-UCLA School of Medicine (United States)
D. Baker M.D., David Geffen-UCLA School of Medicine (United States)
J. Freischlag, Johns Hopkins Univ. School of Medicine (United States)
L. Marcu, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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