
Proceedings Paper
In vitro characterization of corneal wound healing using multiphoton autofluorescence and second harmonic generation (SHG) microscopyFormat | Member Price | Non-Member Price |
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Paper Abstract
The purpose of this investigation is to characterize corneal wound healing under in vitro conditions. Multiphoton
autofluorescence and second harmonic generation (SHG) microscopy will be used to visualize cells and collagen fibers
associated with corneal wound healing. Using the near-infrared excitation source from a titanium-sapphire laser pumped
by a diode-pumped, solid state (DPSS) laser system, we can induce and simultaneously acquire multiphoton
autofluorescence and SHG signals from the cornea specimens. A home-modified commercial microscope system with
specified optical components is used for optimal signal detection. To acquire both high resolution and tissue-level
information of the specimen, a sample positioning stage is used in conjunction with the beam scanning system. Finally,
the organ level image can be assembled from individual area scans. The in vitro samples we used are cornea buttons
acquired from porcine eyes. Localized wounds will be induced by #11 blade and imaged using multiphoton microscopy.
Based on these results, we envision the in vitro imaging chamber to be able to follow the wound healing process without
damaging histological procedures. We envision this approach will enable us to further understand wound healing process
associated with corneal scar and can lead to in vivo methodology for diagnosing cornea damage.
Paper Details
Date Published: 7 March 2006
PDF: 8 pages
Proc. SPIE 6138, Ophthalmic Technologies XVI, 61381U (7 March 2006); doi: 10.1117/12.647413
Published in SPIE Proceedings Vol. 6138:
Ophthalmic Technologies XVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)
PDF: 8 pages
Proc. SPIE 6138, Ophthalmic Technologies XVI, 61381U (7 March 2006); doi: 10.1117/12.647413
Show Author Affiliations
Yen Sun, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Shu-Wen Teng, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Published in SPIE Proceedings Vol. 6138:
Ophthalmic Technologies XVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)
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