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Proceedings Paper

Imaging of skin dermal thermal damage by multiphoton autofluroescence and second harmonic generation (SHG) microscopy
Author(s): Tsung-Lin Yang; Cheng-Tien Chiang; Hsien-Ching Kao; Wen Lo; Sung-Jan Lin; Shiou-Hwa Jee; Yang-Fang Chen; Chen-Yuan Dong
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Paper Abstract

Multiphoton autofluorescence and second harmonic generation (SHG) microscopy are useful in respectively identifying elastin and collagen fibers within the skin dermis. In this study, we attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize the thermal changes to collagen and elastin fibers. We found that autofluorescence and SHG imaging behave differently in skin dermis treated with different temperatures and that an index reflecting the relative changes in autofluorescence and SHG intensity is useful to identify the degree of dermal thermal damage to the skin. With additional development, our approach can be used to identify the extent of thermal damage in patients.

Paper Details

Date Published: 22 February 2006
PDF: 9 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 607805 (22 February 2006); doi: 10.1117/12.647406
Show Author Affiliations
Tsung-Lin Yang, National Taiwan Univ. (Taiwan)
Cheng-Tien Chiang, National Taiwan Univ. (Taiwan)
Hsien-Ching Kao, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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