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Proceedings Paper

In vivo time-resolved autofluorescence measurements on human skin
Author(s): Kamal M. Katika; Laurent Pilon; Katrina Dipple M.D.; Seymour Levin M.D.; Jennifer Blackwell; Halil Berberoglu
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Paper Abstract

In this paper we present preliminary results obtained from fluorescence lifetime measurements on human skin using time-correlated single photon counting (TCSPC) techniques. Human skin was exposed to light from a pulsed LED of 700 ps pulse width at a wavelength of 375 nm and fluorescence decays were recorded at four different emission wavelengths (442, 460, 478 and 496 nm) using a photomultiplier tube (PMT) coupled to a monochromator. Measurements were carried out on the left and right palms of subjects recruited for the study after obtaining consent using a UCLA IRB approved consent form. The subjects recruited consisted of 18 males and 17 females with different skin complexions and ages ranging from 10 to 70 years. In addition, a set of experiments were also performed on various locations including the palm, the arm and the cheek of a Caucasian subject. The fluorescence decays thus obtained were fit to a three-exponential decay model in all cases and were approximately 0.4, 2.7 and 9.4 ns, respectively. The variations in these lifetimes with location, gender, skin complexion and age are studied. It is speculated that the shorter lifetimes correspond to free and bound NADH while the longer lifetime is due to AGE crosslinks.

Paper Details

Date Published: 22 February 2006
PDF: 11 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60780L (22 February 2006); doi: 10.1117/12.647149
Show Author Affiliations
Kamal M. Katika, Univ. of California, Los Angeles (United States)
Laurent Pilon, Univ. of California, Los Angeles (United States)
Katrina Dipple M.D., Univ. of California, Los Angeles (United States)
Seymour Levin M.D., Wadsworth Veterans Administration Hospital (United States)
Jennifer Blackwell, Univ. of California, Los Angeles (United States)
Halil Berberoglu, Univ. of California, Los Angeles (United States)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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