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Proceedings Paper

Monitoring of collagen shrinkage by use of second harmonic generation microscopy
Author(s): Sung-Jan Lin; Jau-Shiuh Chen; Wen Lo; Yen Sun; Wei-Liang Chen; Jung-Yi Chan; Hsin-Yuan Tan; Wei-Chou Lin; Chih-Jung Hsu; Tai-Horng Young; Shiou-Hwa Jee; Chen-Yuan Dong
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Paper Abstract

Thermal treatment induced collagen shrinkage has a great number of applications in medical practice. Clinically, the there is lack of reliable non-invasive methods to quantify the shrinkage. Overt treatment by heat application can lead to devastating results. We investigate the serial changes of collagen shrinkage by thermal treatment of rat tail tendons. The change in length is correlated with the finding in second harmonic generation microscopy and histology. Rat tail tendon shortens progressively during initial thermal treatment. After a certain point in time, the length then remains almost constant despite further thermal treatment. The intensity of second harmonic generation signals also progressively decreases initially and then remains merely detectable upon further thermal treatment. It prompts us to develop a mathematic model to quantify the dependence of collagen shrinkage on changes of SHG intensity. Our results show that SHG intensity can be used to predict the degree of collagen shrinkage during thermal treatment for biomedical applications.

Paper Details

Date Published: 1 March 2006
PDF: 10 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840S (1 March 2006); doi: 10.1117/12.647087
Show Author Affiliations
Sung-Jan Lin, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
National Taiwan Univ. (Taiwan)
Jau-Shiuh Chen, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Jung-Yi Chan, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Cathay General Hospital (Taiwan)
Hsin-Yuan Tan, National Taiwan Univ. (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Wei-Chou Lin, National Taiwan Univ. Hospital (Taiwan)
Chih-Jung Hsu, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Tai-Horng Young, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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