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Proceedings Paper

Monitoring photoaging by use of multiphoton fluorescence and second harmonic generation microscopy
Author(s): Sung-Jan Lin; Shiou-Hwa Jee; Jung-Yi Chan; Ruei-Jr Wu; Wen Lo; Hsin-Yuan Tan; Wei-Chou Lin; Jau-Shiuh Chen; Tai-Horng Young; Chih-Jung Hsu; Chen-Yuan Dong
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Paper Abstract

It is a field of great interest to develop therapies to rejuvenate photoaged skin. However, the treatment response can not be ideally determined due to lack of a reliable non-invasive method to quantify photoaging. In this study, the photoaging process of skin is investigated by use of a multiphoton fluorescence and second harmonic generation microscopy. We obtain the autofluorescence and second harmonic generation images of superficial dermis from facial skin of individuals of different ages. The results show that autofluorescence signals increase with age while second harmonic generation signals decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. In the case of severe photoaging, solar elastosis can be clearly demonstrated by the presence of thick curvy autofluorescent materials in the superficial dermis. We propose a second harmonic generation to autofluorescence aging index of dermis to quantify the photoaging changes. This index is shown to be a good indicator of photoaging. Our results suggest that multiphoton fluorescence and second harmonic generation microscopy can be developed into a non-invasive imaging modelity for the clinical evaluation of photoaging.

Paper Details

Date Published: 22 February 2006
PDF: 7 pages
Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 607803 (22 February 2006); doi: 10.1117/12.647009
Show Author Affiliations
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
Jung-Yi Chan, Cathay General Hospital (Taiwan)
Ruei-Jr Wu, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, National Taiwan Univ. (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Wei-Chou Lin, National Taiwan Univ. (Taiwan)
Jau-Shiuh Chen, National Taiwan Univ. Hospital (Taiwan)
Tai-Horng Young, National Taiwan Univ. (Taiwan)
Chih-Jung Hsu, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, Cathay General Hospital (Taiwan)

Published in SPIE Proceedings Vol. 6078:
Photonic Therapeutics and Diagnostics II
Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Reza S. Malek M.D.; Michael D. Lucroy D.V.M.; Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Justus F. R. Ilgner M.D.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Haishan Zeng; Guillermo J. Tearney M.D.; Bernard Choi, Editor(s)

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