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Proceedings Paper

Molecular differentiation of Leishmania protozoarium using CdS quantum dots as biolabels
Author(s): Beate S. Santos; Patrícia M. A. de Farias; Frederico D. de Menezes; Erick L. Mariano; Ricardo de C. Ferreira; Selma Giorgio; Maira C. Bosetto; Diana C. Ayres; Paulo M. Lima; Adriana Fontes; André A. de Thomas; Carlos L. Cesar
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Paper Abstract

In this work we applied core-shell CdS/Cd(OH)2 quantum dots (QDs) as fluorescent labels in the Leishmania amazonensis protozoarium. The nanocrystals (8-9 nm) are obtained via colloidal synthesis in aqueous medium, with final pH=7 using sodium polyphosphate as the stabilizing agent. The surface of the particles is passivated with a cadmium hydroxide shell and the particle surface is functionalized with glutaraldehyde. The functionalized and non-functionalized particles were conjugated to Leishmania organisms in the promastigote form. The marked live organisms were visualized using confocal microscopy. The systems exhibit a differentiation of the emission color for the functionalized and non-functionalized particles suggesting different chemical interactions with the promastigote moieties. Two photon emision spectra (λexc=795nm) were obtained for the promastigotes labeled with the functionalized QDs showing a significant spectral change compared to the original QDs suspension. These spectral changes are discussed in terms of the possible energy deactivation processes.

Paper Details

Date Published: 10 February 2006
PDF: 9 pages
Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 609706 (10 February 2006); doi: 10.1117/12.646912
Show Author Affiliations
Beate S. Santos, Univ. Federal de Pernambuco (Brazil)
Patrícia M. A. de Farias, Univ. Federal de Pernambuco (Brazil)
Frederico D. de Menezes, Univ. Federal de Pernambuco (Brazil)
Erick L. Mariano, Univ. Federal de Pernambuco (Brazil)
Ricardo de C. Ferreira, Univ. Federal de Pernambuco (Brazil)
Selma Giorgio, Univ. Estadual de Campinas (Brazil)
Maira C. Bosetto, Univ. Estadual de Campinas (Brazil)
Diana C. Ayres, Univ. Estadual de Campinas (Brazil)
Paulo M. Lima, Univ. Estadual de Campinas (Brazil)
Adriana Fontes, Univ. Estadual de Campinas (Brazil)
André A. de Thomas, Univ. Estadual de Campinas (Brazil)
Carlos L. Cesar, Univ. Estadual de Campinas (Brazil)

Published in SPIE Proceedings Vol. 6097:
Optical Molecular Probes for Biomedical Applications
Samuel I. Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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