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Proceedings Paper

Multimodal microscopy of immune cells and melanoma for longitudinal studies
Author(s): David Entenberg; Iana Aranda; Yongbiao Li; Ricardo Toledo-Crow; David Schaer; Yanyun Li
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Paper Abstract

Intravital microscopy of cancer is a well established tool that provides direct visualization of the tumor cycle. It traditionally involves one of several strategies: invasive subcutaneous (SC) implantation of tumors followed by surgical opening of skin flaps for imaging, techniques utilizing skin fold chambers and implanted optical windows or intradermal injections under 200μm from the skin surface. All of these techniques allow the use of fluorescent proteins as markers for biologically significant constituents. However, observation methods utilizing skin-flaps, skin-fold chambers and optical windows are invasive and tend to alter the immune environment of the tissue and/or limit the duration of studies that can be performed. If implanted correctly, intradermally injected tumors can be minimally invasive, will not require biopsies or surgical intervention to observe and are accessible for direct transdermal imaging with a number of in vivo modalities. We present our work in the development of a small animal intravital microscopy workstation that allows the acquisition of different contrast imaging modalities: reflectance confocal, wide field epifluorescence, multiphoton and second harmonic generation (SHG). The images are acquired pair-wise simultaneously and sequentially in time. The aim of our instrumentation is to gather all information generated by the single probing beam via the reflected or back-scattered signal, SHG signal and various fluorescence signals. Additionally, we also present our development of a microscopic tissue navigation technique to mark, label and track sites of interest. This technique enables us to revisit sites periodically and record, with different imaging contrasts, their biological changes over time.

Paper Details

Date Published: 13 February 2006
PDF: 12 pages
Proc. SPIE 6081, Multimodal Biomedical Imaging, 60810A (13 February 2006); doi: 10.1117/12.646907
Show Author Affiliations
David Entenberg, Memorial Sloan-Kettering Cancer Ctr. (United States)
Iana Aranda, Memorial Sloan-Kettering Cancer Ctr. (United States)
Yongbiao Li, Memorial Sloan-Kettering Cancer Ctr. (United States)
Ricardo Toledo-Crow, Memorial Sloan-Kettering Cancer Ctr. (United States)
David Schaer, Memorial Sloan-Kettering Cancer Ctr. (United States)
Yanyun Li, Memorial Sloan-Kettering Cancer Ctr. (United States)

Published in SPIE Proceedings Vol. 6081:
Multimodal Biomedical Imaging
Fred S. Azar; Dimitris N. Metaxas, Editor(s)

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